Streptavidin-enhanced assay for sensitive and specific detection of single nucleotide polymorphism in TP53
Identifieur interne : 002684 ( Main/Exploration ); précédent : 002683; suivant : 002685Streptavidin-enhanced assay for sensitive and specific detection of single nucleotide polymorphism in TP53
Auteurs : Hana Šípová [République tchèque] ; Tomáš Špringer [République tchèque] ; Ji Homola [République tchèque]Source :
- Analytical and Bioanalytical Chemistry [ 1618-2642 ] ; 2011-03-01.
English descriptors
- KwdEn :
Abstract
Abstract: This paper reports an approach to detection of single nucleotide polymorphism based on special amplification assay and surface plasmon resonance biosensor technology. In this assay, a part of the target DNA is recognized by a probe (probe A) coupled with streptavidin–oligonucleotide (SON) complexes ex situ, and when the mixture is injected in the sensor, another part of the target DNA is recognized by a DNA probe (probe B) immobilized on the sensor surface. To achieve high sensitivity and specificity, the assay is optimized in terms of composition of SON complexes, probe design, and assay temperature. It is demonstrated that this approach provides high specificity (no response to targets containing single-mismatched bases) and sensitivity (improves sensor response to perfectly matched oligonucleotides by one order of magnitude compared to the direct detection method). The assay is applied to detection of a short synthetic analogue of TP53 containing a “hot spot”—single nucleotide mismatch frequently mutated in germ line cancer—at levels down to 40 pM.
Url:
DOI: 10.1007/s00216-010-3863-9
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001147
- to stream Istex, to step Curation: 001147
- to stream Istex, to step Checkpoint: 000555
- to stream Main, to step Merge: 002709
- to stream Main, to step Curation: 002684
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Streptavidin-enhanced assay for sensitive and specific detection of single nucleotide polymorphism in TP53</title><author><name sortKey="Sipova, Hana" sort="Sipova, Hana" uniqKey="Sipova H" first="Hana" last="Šípová">Hana Šípová</name></author><author><name sortKey="Springer, Tomas" sort="Springer, Tomas" uniqKey="Springer T" first="Tomáš" last="Špringer">Tomáš Špringer</name></author><author><name sortKey="Homola, Ji" sort="Homola, Ji" uniqKey="Homola J" first="Ji" last="Homola">Ji Homola</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:AFB04E0BA8DFE2F39108CC70B2A6996AFAB64D51</idno><date when="2010" year="2010">2010</date><idno type="doi">10.1007/s00216-010-3863-9</idno><idno type="url">https://api.istex.fr/ark:/67375/VQC-JTTH5JBS-Q/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001147</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001147</idno><idno type="wicri:Area/Istex/Curation">001147</idno><idno type="wicri:Area/Istex/Checkpoint">000555</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000555</idno><idno type="wicri:doubleKey">1618-2642:2010:Sipova H:streptavidin:enhanced:assay</idno><idno type="wicri:Area/Main/Merge">002709</idno><idno type="wicri:Area/Main/Curation">002684</idno><idno type="wicri:Area/Main/Exploration">002684</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Streptavidin-enhanced assay for sensitive and specific detection of single nucleotide polymorphism in TP53</title><author><name sortKey="Sipova, Hana" sort="Sipova, Hana" uniqKey="Sipova H" first="Hana" last="Šípová">Hana Šípová</name><affiliation wicri:level="3"><country xml:lang="fr">République tchèque</country><wicri:regionArea>Institute of Photonics and Electronics, Academy of Sciences of the Czech Republic, Chaberská 57, 182 51, Prague</wicri:regionArea><placeName><settlement type="city">Prague</settlement><region type="région" nuts="2">Bohême centrale</region></placeName></affiliation></author><author><name sortKey="Springer, Tomas" sort="Springer, Tomas" uniqKey="Springer T" first="Tomáš" last="Špringer">Tomáš Špringer</name><affiliation wicri:level="3"><country xml:lang="fr">République tchèque</country><wicri:regionArea>Institute of Photonics and Electronics, Academy of Sciences of the Czech Republic, Chaberská 57, 182 51, Prague</wicri:regionArea><placeName><settlement type="city">Prague</settlement><region type="région" nuts="2">Bohême centrale</region></placeName></affiliation></author><author><name sortKey="Homola, Ji" sort="Homola, Ji" uniqKey="Homola J" first="Ji" last="Homola">Ji Homola</name><affiliation wicri:level="3"><country xml:lang="fr">République tchèque</country><wicri:regionArea>Institute of Photonics and Electronics, Academy of Sciences of the Czech Republic, Chaberská 57, 182 51, Prague</wicri:regionArea><placeName><settlement type="city">Prague</settlement><region type="région" nuts="2">Bohême centrale</region></placeName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">République tchèque</country></affiliation></author></analytic><monogr></monogr><series><title level="j">Analytical and Bioanalytical Chemistry</title><title level="j" type="abbrev">Anal Bioanal Chem</title><idno type="ISSN">1618-2642</idno><idno type="eISSN">1618-2650</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="2011-03-01">2011-03-01</date><biblScope unit="volume">399</biblScope><biblScope unit="issue">7</biblScope><biblScope unit="page" from="2343">2343</biblScope><biblScope unit="page" to="2350">2350</biblScope></imprint><idno type="ISSN">1618-2642</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1618-2642</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amplification</term><term>Point mutation</term><term>Single nucleotide polymorphism</term><term>Surface plasmon resonance</term><term>TP53</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: This paper reports an approach to detection of single nucleotide polymorphism based on special amplification assay and surface plasmon resonance biosensor technology. In this assay, a part of the target DNA is recognized by a probe (probe A) coupled with streptavidin–oligonucleotide (SON) complexes ex situ, and when the mixture is injected in the sensor, another part of the target DNA is recognized by a DNA probe (probe B) immobilized on the sensor surface. To achieve high sensitivity and specificity, the assay is optimized in terms of composition of SON complexes, probe design, and assay temperature. It is demonstrated that this approach provides high specificity (no response to targets containing single-mismatched bases) and sensitivity (improves sensor response to perfectly matched oligonucleotides by one order of magnitude compared to the direct detection method). The assay is applied to detection of a short synthetic analogue of TP53 containing a “hot spot”—single nucleotide mismatch frequently mutated in germ line cancer—at levels down to 40 pM.</div></front></TEI><affiliations><list><country><li>République tchèque</li></country><region><li>Bohême centrale</li></region><settlement><li>Prague</li></settlement></list><tree><country name="République tchèque"><region name="Bohême centrale"><name sortKey="Sipova, Hana" sort="Sipova, Hana" uniqKey="Sipova H" first="Hana" last="Šípová">Hana Šípová</name></region><name sortKey="Homola, Ji" sort="Homola, Ji" uniqKey="Homola J" first="Ji" last="Homola">Ji Homola</name><name sortKey="Homola, Ji" sort="Homola, Ji" uniqKey="Homola J" first="Ji" last="Homola">Ji Homola</name><name sortKey="Springer, Tomas" sort="Springer, Tomas" uniqKey="Springer T" first="Tomáš" last="Špringer">Tomáš Špringer</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002684 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002684 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:AFB04E0BA8DFE2F39108CC70B2A6996AFAB64D51
|texte= Streptavidin-enhanced assay for sensitive and specific detection of single nucleotide polymorphism in TP53
}}
| This area was generated with Dilib version V0.6.33. |  |